INT (chemical)

INT (iodonitrotetrazolium or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium) is a commonly used tetrazolium salt (usually prepared with chloride ions), similar to tetrazolium chloride that on reduction produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.[1]

INT
Iodonitrotetrazolium chloride.svg
Names
IUPAC name
3-(4-Iodophenyl)-2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium chloride
Other names
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
Identifiers
CAS Number
  • 146-68-9
3D model (JSmol)
  • Interactive image
ChemSpider
  • 58482
ECHA InfoCard100.005.161 Edit this at Wikidata
PubChem CID
  • 629284
CompTox Dashboard (EPA)
  • DTXSID30932744 Edit this at Wikidata
InChI
  • InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
    Key: JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES
  • [Cl-].[O-][N+](=O)c1ccc(cc1)n3nc(n[n+]3c2ccc(I)cc2)c4ccccc4
Properties
Chemical formula
C19H13ClIN5O2
Molar mass505.70 g·mol−1
Melting point240 °C (464 °F; 513 K) (decomposes)
Solubility in methanol: water (1:1)50 mg/mL hot, very faintly turbid, very deep yellow
log P−2.4
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references


INT is an artificial electron acceptor which can be utilized in a colorimetric assay to determine the concentration of protein in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red. 


This article uses material from the Wikipedia article
 Metasyntactic variable, which is released under the 
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Attribution-ShareAlike 3.0 Unported License.